Looking at Histology slides

 We have some human lymph node slides. Examine these under the microscope and make a drawing under low power and high power.  Label the parts of the lymph node as described in this excellent website

https://histologyblog.com/2012/04/30/histoquarterly-lymph-node/\

These are numbers 16, 19 in the slide A box 

Examine Human blood smear and identify the RBC and WBC , try to identify WBC's using the 

American Hematology Atlas

Articles on H5N1

 Dr. Filiatrault was a co-author of this article . Read it and summarize it 

Opinion: Gambling with bird flu is asking for trouble — and another pandemic

A TALE OF TWO CITIES: SARS outbreak in Vancouver and Toronto

 Read about it here

https://www.archives.gov.on.ca/en/e_records/sars/report/v2-pdf/Vol2Chp3iii.pdf

Interview with the Dr. who stopped SARS

read the following newspaper article written by Pete McMartin in 2010, in the Vancouver Sun :

A VANCOUVER DR WHO STOPPED SARS COLD

By Pete McMartin, Vancouver Sun May 15, 2010 

On March 7, 2003, at 1:55 p.m., Dr. Lyne Filiatrault was nearing the end of her shift when a 55-year-old man was wheeled into the emergency department of Vancouver General Hospital. He was turning blue.

That day he had returned from a visit to Hong Kong, where he and his wife had stayed on the 14th floor of the Metropole Hotel. Staying on the ninth floor of the same hotel was a physician from mainland China, who, the week the man and his wife were there, developed symptoms so debilitating that he had checked himself into a Hong Kong hospital on Feb. 22. There he was put into an intensive care unit. He died on March 4.

The illness the Chinese physician died of was so new to the medical community it did not yet have a name, but within days it would come to be known as Severe Acute Respiratory Syndrome. SARS.

From that ninth floor of the Metropole, the disease spread outward, like ripples from a stone dropped in water. A second Metropole guest died on March 13 after travelling to Vietnam. Health care workers there were infected, as were medical staff in Hong Kong, probably because they had not taken the correct respiratory precautions. Cases were reported in Singapore, Taiwan, Thailand, the Philippines and China. Fears grew that a new global pandemic was gaining momentum.

But on March 7, no one in the emergency department of VGH knew what SARS was. The BC Centre for Disease Control had sent out universal e-mails warning hospital staffs to be on the lookout for a new strain of avian flu, but SARS is not avian flu, and e-mails can go unread.

Filiatrault, however, was the kind of doctor who read all her e-mails, especially those from the BCCDC. She has a reputation for a fanatical sense of duty and hard work, often working hours past her shift's end.

"She works so hard and is so dedicated to her work," said her husband, Dr. David Haughton, an emergency-department physician at Children's Hospital, "that she'll wake up in the middle of the night because she's worried she forgot some detail, and then she'll phone the ward. She's done it so many times, they'll just pick up the phone and say, 'Lyne, go back to sleep.'"

So while Filiatrault did not know exactly what the man who came through VGH's doors on March 7 ailed from, she knew enough from reading the BCCDC's e-mails that a patient recently arrived from Asia who was having such trouble breathing that he was turning blue warranted medical care of extraordinary caution.

Often, emergency-ward patients in a teaching hospital will first be examined by students or interns, but Filiatrault, who had worked in VGH's emergency department since 1997, examined the patient herself. She summoned a respirologist. The patient was immediately put on oxygen and blood work was done. She, the attending nurse and the respirologist hoisted the patient into bed.

"Initially, when he came in," Filiatrault said, "we didn't have any protective gear, so we were lucky we didn't contract the disease. I came down with a mild flu that weekend, which freaked my family out, but I was tested, and I never developed any antibodies."

Within minutes, it was decided that the man, who would soon become known as Patient Zero, should be placed in isolation. It was a group decision made by the department personnel, Filiatrault said, not her alone.

"I don't care to, or want to, take sole responsibility for the decisions that were made concerning the patient," she said. "I can't stress enough that this was a group effort."

It should be interjected here that Filiatrault is so self-effacing she originally did not want to do an interview, and had to be persuaded to do so by her husband and VGH media-relations staff. The part she played in containing the SARS outbreak in Vancouver, as far as I could discern, had never been told in the media before because she had never spoken to the media about it. Only her peers knew of it. Even in talking to us, she played it down.

"Like I said, the stars were aligned and it just worked well. We were lucky. It could have gone the other way. We could have been infected but weren't. And in 2003, we had the luxury of no crowding and an isolation room that was immediately available."

Her peers have a different view. Dr. Roy Purssell, who was head of the emergency department at the time, wasn't surprised at her version. "She played it down, didn't she?" he asked, as if he expected her to. "But what was done ... she handled it with just an incredible attention to detail. It all got handled just as it should be, with meticulous care and great communication between staff. Lives were saved, and the economic impact that this could have had in B.C. was avoided. In Toronto, it cost the city ... what? ... over a billion dollars? I'm very proud of her, and proud to be working with her."

In Toronto, where another infected patron of the Metropole Hotel returned on Feb. 23, infecting her son before dying at home on March 5, the SARS epidemic breached the hospitals' first line of defence, to disastrous results.

Ontario did not have an equivalent to the BCCDC, and so did not have a centralized warning system for communicable diseases. When Ontario's Patient Zero presented himself to an emergency ward on March 7, the same day our Patient Zero arrived at VGH, he was thus not recognized as a special threat and was placed in general observation in an emergency room for 18 hours. He was not placed in isolation until 21 hours after arriving at the hospital.

By then it was too late. Fortyfour people would die in Ontario from SARS, thousands would be placed in quarantine and the cost just to the Ontario business economy was estimated in the billions.

Here in B.C., there were five reported cases of SARS. All of them recovered, including Patient Zero.

Filiatrault, however, counselled caution. (And it was the following that she insisted the story concentrate on.)

"In 2003, as I said, we had the luxury of an isolation room that was immediately available to us.

"Nowadays? The number-one complaint in patient surveys? Wait times. And the wait times are too long. Nowadays, what happens with the overcrowding in emergency departments is, people wait for hours in triage. If somebody has a sprained ankle, fine, they can sit there for five hours. But if somebody has a serious communicable disease, they can sit next to people for hours without us knowing it, and expose them."

This is a complaint emergency-room doctors have been taking public. There has been a recent campaign to get the provincial government to pony up another $10 million, to hire another 35 emergency physicians to cope with the 25-per-cent increase in emergency-room traffic at our larger hospitals.

"We're the safety net," Filiatrault said, "and really, if there are not enough strands, it affects your care. In the future, I think we're at the risk of potentially having people infected."

A postscript to this story:

For its work in containing the SARS outbreak in those first days of March 2003, the VGH emergency department received a special award from the province. Filiatrault was asked to speak at the award ceremony to describe the events that day when Patient Zero came through the doors.

She declined, and asked Purssell to speak in her place.

She worked her shift, instead.

pmcmartin@vancouversun.com 604-605-2905

© Copyright (c) The Vancouver Sun

Read more: http://www.vancouversun.com/opinion/...#ixzz0o0sVGGUt

Bacteria lab

   

 HYPOTHESIS:

Create a hypothesis about what area of the school will yield the fastest growing bacteria and the most number of colonies. What factors do you think led to growing more bacteria or more different types?

PROCEDURE:

Use agar plates and q-tips to sample different surfaces around our school.  Store the plates UPSIDEDOWN in a secure location in the classroom.  Note where you found the sample

OBSERVATION
 Next, create a data table showing in the following columns

1.  names
2.  a. location of sample
2.b. is this a high traffic location for human contact?
2c.  is this a dry, sticky, wet, warm, cold location, or don't know?

GROW THE BACTERIA

3.a count the number of colonies
3b.  and their relative size of colonies (estimate size of each dot or circle diameter based on petri dish size
4.  colour of colonies
5.  Any mold sample?

Note that a nil result tells you something too!


ANALYSIS
1. Tell me some patterns that you are noticing in the collection of these samples.
2. Which locations are yielding the most samples.
3.Why might some samples yield more bacteria or faster growing bacteria?
4. What are some limitations of this study and some sources of experimental error?

CONCLUSION
Write a well reasoned conclusion based on your observations.

EVALUATION   IS OUT OF 25 IN TOTAL ...create a ppt for this assignment!

5

presentation is out of 5.  for 5/5 it is typed  with illustrations or photos and emailed to for 4/5 it is pretty good but there are a few spelling mistakes.  for 0-3 out of 5 it is in pencil or messy.  

10

Clear writing is out of 10.  for 9/10 or 10/10, it is written with no English mistakes and it clearly communicates each part of the lab report.    7-8/10  it is very well written     for 5-6/10 some parts are not so clear and the reader must guess what you are trying to say.  0-4/10, it is minimally communicating what happened

10

Paragraphs are insightful and your observations are well done. this is out of 10.

             9-10/10,   your observations and conclusions are well written and logical.  It is brilliant

              7-8/10   your observations and conclusions are very well done

               5-6   your conclusions don't really logically follow from your results

                0-4   your conclusions are not relating to your results at all

DISEASE VECTOR Assignments

 SLIDESHOW ON DISEASE

Read and analyze the following scenarios :  

CENTRE FOR DISEASE CONTROL  CASE STUDIES                             

  

CREATE DISEASE SUMMARY SHEETS WHICH INCLUDES THE FOLLOWING INFORMATION in pen. Each worth 15 marks total of 45 marks.  You may work in a group. 

1. case study: covid 19 on a cruise ship in Japan
article on cruise ship in Japan

https://www.forbes.com/sites/victoriaforster/2020/02/07/coronavirus-outbreak-on-quarantined-japan-cruise-ship-spreads-61-passengers-now-affected/#21bb8de328c4

2. case study: Ebola outbreak in North America
watch the video first

 https://m.youtube.com/watch?v=XCrOde-JYs0

here is the case study: https://www.google.com/amp/s/abcnews.go.com/amp/Health/ebola-america-timeline/story%3fid=26159719

3.  Case study: Measles in BC, a resurgence of an old disease

https://vancouversun.com/news/local-news/measles-in-b-c-how-we-got-here-and-what-you-need-to-know

and a summary of BC CDC response:

https://www.google.com/url?sa=t&source=web&rct=j&url=http://www.health.gov.bc.ca/library/publications/year/2019/measles-update-may2019.pdf&ved=2ahUKEwi1yvrT9r7uAhVLHzQIHbIQCLoQFjACegQICRAC&usg=AOvVaw3B1SCyd7RHx_OoGIMdFUln

The case of Avian Influenza infecting Dairy Cows

https://www.cbc.ca/news/health/bird-flu-in-u-s-cows-caught-scientists-by-surprise-canadian-research-has-seen-it-coming-since-1953-1.7212587

CENTRE FOR DISEASE CONTROL             

NAMES of group_________________

Name of disease:

Symptoms of the disease: ( 2 marks )

Age, sex and other details of the certain victims who are mentioned in the articles above 

 ( 3 marks )

Possible disease vectors, consider multiple vectors:  (2 marks)             

         Food

         Water

         Aerosol

         Soil

         Animal

         Human contact

                  Blood, mucous, saliva,

                  Feces, sexual contact 

Give  Evidence of this disease vectors                                                                     
(3 marks)

List the major events of the outbreak and the response of CDC in the order that it took place:  

Using drawings in ink and colour:  Number the events and highlight the events.  List measures taken  to contain the outbreak successfully. 

(5 marks)

Introduction to Microbiology: Viruses

 This is an Introduction to Microbiology Notes, which cover Virus, and Monerans, with a mention of unicellular fungi.  Read this document as an overview. 

Read this Introduction to Viruses and also find out how Viruses can infect their hosts

Viruses can infect cells using the LYTIC CYCLE.  

Review the 

STEPS TO THE LYTIC CYLE: memorize AESAR

• Attachment
• Entry & degradation of host's DNA
• Synthesis of new viruses 
• duplication of virus' genetic material
• creation of new virus parts
• Assembly of parts into new viruses
• Release of new viruses

 video shows lytic cycle

COVID-19 is a virus that undergoes a lytic cycle, but it does not explode cells, instead, it simply gets released from a cell